The OECD has released two new guidelines for eye irritation testing using in vitro methods. OECD TG 491 covers the Short Time Exposure In Vitro Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage. The Short Time Exposure (STE) assay utilizes a rabbit corneal cell line to determine if a test substance has the potential to cause serious eye damage or if it is sufficiently mild to qualify for "no classification" labeling. The STE assay can be used in both a top-down and bottom-up tiered testing approach.
OECD TG 492 covers the use of the Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals Not Requiring Classification and Labeling for Eye Irritation or Serious Eye Damage. This covers the EpiOcular Eye Irritation Test (EIT) which uses a commercially available, 3D ocular tissue construct to determine the irritation potential of an undiluted test substance.
Please contact IIVS to see how these methods can be used in a tiered testing program for your materials. For additional information on the new OECD test guidelines and a link to a recent IIVS webinar overview of a number of in vitro assays, please follow the link above.